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Differential effects of Alloherpesvirus CyHV-3 and Rhabdovirus SVCV on apoptosis in fish cells

Differential effects of Alloherpesvirus CyHV-3 and Rhabdovirus SVCV on apoptosis in fish cells

Miest, Joanna Junack ORCID: 0000-0003-2534-0530, Adamek, Mikolaj, Pionnier, Nicolas, Harris, Sarah, Matras, Marek, Rakus, Krzysztof L., Irnazarow, Ilgiz, Steinhagen, Dieter and Hoole, Dave (2015) Differential effects of Alloherpesvirus CyHV-3 and Rhabdovirus SVCV on apoptosis in fish cells. Veterinary Microbiology, 176 (1-2). pp. 19-31. ISSN 0378-1135 (Print), 1873-2542 (Online) (doi:10.1016/j.vetmic.2014.12.012)

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Abstract

Whilst Herpesviridae, which infect higher vertebrates, actively influence host immune responses to ensure viral replication, it is mostly unknown if Alloherpesviridae, which infect lower vertebrates, possess similar abilities. An important antiviral response is clearance of infected cells via apoptosis, which in mammals influences the outcome of infection. Here, we utilise common carp infected with CyHV-3 to determine the effect on the expression of genes encoding apoptosis-related proteins (p53, Caspase 9, Apaf-1, IAP, iNOS) in the pronephros, spleen and gills. The influence of CyHV-3 on CCB cells was also studied and compared to SVCV (a rhabdovirus) which induces apoptosis in carp cell lines. Although CyHV-3 induced iNOS expression in vivo, significant induction of the genetic apoptosis pathway was only seen in the pronephros. In vitro CyHV-3 did not induce apoptosis or apoptosis-related expression whilst SVCV did stimulate apoptosis. This suggests that CyHV-3 possesses mechanisms similar to herpesviruses of higher vertebrates to inhibit the antiviral apoptotic process.

Item Type: Article
Additional Information: © 2015 This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords: CyHV-3; KHV; SVCV; CCB; Apoptosis; Alloherpesviridae
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Life & Sports Sciences
Last Modified: 07 Apr 2017 20:26
Selected for GREAT 2016: GREAT a
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/15083

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