Skip navigation

Development of flavonoid therapeutics: Transport mechanisms in the model eukaryote Dictyostelium discoideum

Development of flavonoid therapeutics: Transport mechanisms in the model eukaryote Dictyostelium discoideum

Thompson, Elinor and Ferrara, Billy (2016) Development of flavonoid therapeutics: Transport mechanisms in the model eukaryote Dictyostelium discoideum. In: The Microbiology Society annual conference, 21-24 March 2016, Liverpool, UK.

[img] PDF (Poster abstract)
15081_Thompson_Development of flavonoid therapeutics (abstract) 2016.pdf - Accepted Version
Restricted to Registered users only

Download (57kB)
[img] PDF (Publisher PDF)
15081_Thompson_Poster abstract book (pub PDF) 2016.pdf - Published Version
Restricted to Registered users only

Download (1MB)

Abstract

We are investigating the function of two members of the multidrug and toxin efflux (MATE) transporter family, which are ubiquitous throughout all living kingdoms. MATEs have a wide range of substrate targets, with transporters conferring the ability to facilitate the movement of single or many compounds (Omote et al., 2006).

We have identified two genes encoding 'MATE' proteins in the model amoeba Dictyostelium discoideum, and are making knockout and reporter lines for physiological and imaging studies. Expression of the transporters at different life stages suggests the two have distinct and not redundant functions. The first transporter gene is transcribed in highest levels when cells signal to each other and aggregate. The second transporter gene is predominant earlier, when unicellular Dictyostelium cells prey on bacteria, and transcription also peaks later when cells have aggregated to form a motile, multicellular slug.

We hope to address fundamental questions in the cell biology of this important model organism such as the role of the extracellular matrix and how it is formed; to study localisation of these proteins in the unicellular and multicellular life cycle; and also to contribute to work on the mechanisms of a flavonoid therapy for polycystic kidney disease which is under development by our collaborators (Waheed et al., 2014). In plants, it is hypothesised that so-called 'MATE' transporter proteins may be involved in flavonoid transport: currently there is sparse literature on the transport mechanisms that would allow/prevent this family of compounds reaching eukaryotic cellular targets.

Item Type: Conference or Conference Paper (Poster)
Uncontrolled Keywords: flavonoid, kidney disease, therapeutic, Dictyostelium, model microbe
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Department of Life & Sports Sciences
Last Modified: 14 Jun 2017 13:22
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/15081

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics