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High-resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus

High-resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus

Bayer, Philipp E., Ruperao, Pradeep, Mason, Annaliese S., Stiller, Jiri, Chan, Chon-Kit Kenneth, Hayashi, Satomi, Long, Yan, Meng, Jinling, Sutton, Tim, Visendi, Paul, Varshney, Rajeev K., Batley, Jacqueline and Edwards, David (2015) High-resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus. Theoretical and Applied Genetics, 128 (6). pp. 1039-1047. ISSN 0040-5752 (Print), 1432-2242 (Online) (doi:10.1007/s00122-015-2488-y)

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Abstract

The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene–trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.

Item Type: Article
Additional Information: The final publication is available at Springer via http://dx.doi.org/10.1007/s00122-015-2488-y
Uncontrolled Keywords: Double Haploid; Conversion Processes; Supplementary Table; Recombination Bin; Recombination Map; Genome-wide Recombination; Reference Genome; Reference Cultivar; Reference Genome Sequence; Black Gram Husk; Recombinant Inbred Line; F2 Individual; Heterozygous SNPs; Recombination Hotspot
Faculty / Department / Research Group: Faculty of Engineering & Science > Natural Resources Institute > Agriculture, Health & Environment Department
Last Modified: 12 Oct 2016 14:40
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/14760

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