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Isolation and expression of tilapia (Oreochromis niloticus) serine 8-type GnRH coding and regulatory sequences

Isolation and expression of tilapia (Oreochromis niloticus) serine 8-type GnRH coding and regulatory sequences

Rahman, M. Azizur, Farahmand, Hamid, Sohm, Frederic, Hwang, Gwu-Lyn and Maclean, Norman (2003) Isolation and expression of tilapia (Oreochromis niloticus) serine 8-type GnRH coding and regulatory sequences. Gene, 304. pp. 97-106. ISSN 0378-1119 (Print), 1879-0038 (Online) (doi:10.1016/S0378-1119(02)01184-8)

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Abstract

The complete Serine 8-type gonadotropin releasing hormone (GnRH) coding sequence with a substantial 5-prime regulatory sequence (5 kb) has been isolated and characterised in Nile Tilapia (Oreochromis niloticus) from a relevant genomic library. The primary structure of the protein precursor was identified for this gene. The promoter efficacy has been tested using 0.6 kb of the GnRH promoter driving a lacZ reporter gene in both cultured spleen cells and transiently expressing zebrafish. In the cell transfection experiments, the average level of β-galactosidase activity in transfected cells was more than 2.1 (P<0.05) times higher than the control promoter-less vector in five independent cultures indicating that the 0.6GnRH/lacZ construct is able to express in spleen cells. In addition, the transient expression of the lacZ gene was detected in the brain of G0 zebrafish embryos (Danio rerio) 4 days after fertilisation following egg injection with the construct, which demonstrated the efficacy of the tilapia GnRH promoter.

Item Type: Article
Uncontrolled Keywords: Serine 8-type gonadotropin releasing hormone; Promoter; Preprohormone; Transgenic fish; Tilapia (Oreochromis niloticus); Zebrafish (Danio rerio)
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Life & Sports Sciences
Last Modified: 17 Oct 2016 09:14
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/14504

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