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Having a blast with gene transfer

Having a blast with gene transfer

Pecorino, Lauren T. and Lo, Donald C. (1992) Having a blast with gene transfer. Current Biology, 2 (1). pp. 30-32. ISSN 0960-9822 (Print), 1879-0445 (Online) (doi:10.1016/0960-9822(92)90421-6)

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Abstract

The ability to introduce cloned genes into cells, generally referred to as transformation or transfection; is one of the most powerful and far-reaching methodologies to come out of~molecular biology. It has played a critical role in the study of gene expression and protein stucture and function. However, many standard techniques work on only limited ranges of host cells and others are labor intensive or require large numbers of cells. Enter ‘biolistics’ (biological ballistics), a relatively new method of transfection that literally shoots high velocity microprojectiles, carrying DNA, into cells. It promises to be an efficient transfection method with much broader applicability.
The biolistic process (for review see [1,2 3 > was originally developed by Sanford et al. [3] as a means of introducing DNA into plant cells. The limitations of existing methods of gene transfer stimulated the idea of shooting tungsten or gold particles coated with DNA directly into cells. Since then its use in transfection has extended well beyond plants to an ever-growing list of cell types, some of which had previously been recalcitrant to more routine methods of gene transfer.

Item Type: Article
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Life & Sports Sciences
Last Modified: 17 Oct 2016 09:13
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/14222

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