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Dictyostelium discoideum: a model for testing novel inhibitors of urokinase-type plasminogen activator

Dictyostelium discoideum: a model for testing novel inhibitors of urokinase-type plasminogen activator

Thompson, Elinor (2013) Dictyostelium discoideum: a model for testing novel inhibitors of urokinase-type plasminogen activator. In: Poster Society for General Microbiology (SGM) Autumn meeting 2013, September 2013, Warwick.

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Abstract

The social amoeba Dictyostelium discoideum is a useful non-animal eukaryote for testing novel compounds and dissecting cell regulatory molecular networks. We used this model organism to investigate the effect of a series of arylboronic acids and pinacol esters on development, chemotaxis and viability. These compounds were studied in parallel by collaborators for serine protease and urokinase-type plasminogen activator (uPA) inhibition, both in vitro and in vivo. In those biochemical assays, three compounds, BC11, SR3 and BC57, displayed micromolar (50 µM) inhibition of uPA, with an excellent selectivity profile over related proteases (Smith et al., 2012). Notably, the same compounds disturbed cell adhesion and migration in Dictyostelium, without any effect on viability. Compound BC11 was chemotoxic rather than just chemostatic, proving to be the most potent and selective inhibitor of uPA in these independent Dictyostelium and biochemical assays.

Smith E, Spencer J, Ali M, et al. (2012) Elucidating novel urokinase-type plasminogen activator inhibitors. In Journal of Thrombosis and Haemostasis, ppe10–e24.

Item Type: Conference or Conference Paper (Poster)
Uncontrolled Keywords: Dictyostelium; inhibitor
Subjects: Q Science > QR Microbiology
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Life & Sports Sciences
Last Modified: 17 Oct 2016 09:13
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/13993

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