Skip navigation

Regulation of mitochondria by proteolysis

Regulation of mitochondria by proteolysis

Thompson, Elinor (2015) Regulation of mitochondria by proteolysis. In: Society for General Microbiology (SGM) Annual Conference 2015, 30 March - 2 April 2015, Birmingham.

[img]
Preview
PDF (Author's Accepted Manuscript)
13991_THOMPSON_Regulation_Proteolysis_2015.pdf - Accepted Version

Download (71kB)

Abstract

Proteolysis is increasingly documented as a method of regulation of mitochondrial function. Our studies of rhomboid-family proteins' roles in organelles show that this is also the case in the social amoeba Dictyostelium discoideum, in which four of these membrane-bound, evolutionarily ubiquitous, serine proteases are found. Rhomboid proteases act on disparate substrates in different organisms so far studied, but their mode of action is conserved: their location in the membrane means that their membrane-tethered substrates can act in signalling upon release, or be activated, by rhomboid-mediated cleavage. Among eukaryotic rhomboids is the mitochondrial protease 'PARL', which ensures the maintenance of the structural and functional integrity of mitochondria and plastids, but we have found that other Dictyostelium rhomboids also affect the organelle. Studying the development and behaviour of Dictyostelium, a microbial model organism with a complex life cycle that includes uni- and multicellular stages, allowed investigation of the role of rhomboids in unicellular vegetative growth, multicellular development and sporulation, phagocytosis, and response to the environment. We found that two rhomboid-null mutants gave rise to changes in development, rhmA altering the response to chemoattractants and demonstrating decreased motility in general, whereas rhmB null cells had slower growth rates with decreased response to folic acid. RhmA, although located in the contractile vacuole, affects the ultrastructure of mitochondria, and RhmB-GFP fusions protein was localised to the mitochondrion. qPCR analysis revealed RhmA and RhmB transcript levels peaking during the multicellular growth phase and transcriptional networks suggest the Dictyostelium rhmA is regulated along with the orthologues of Saccharomyces cerevisiae mitochondrial rhomboid substrates.

Item Type: Conference or Conference Paper (Lecture)
Uncontrolled Keywords: Dictyostelium, rhomboid, protease, membrane
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Life & Sports Sciences
Last Modified: 17 Oct 2016 09:13
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/13991

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics