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Probing the interaction of nanoparticles with mucin for drug delivery applications using dynamic light scattering

Probing the interaction of nanoparticles with mucin for drug delivery applications using dynamic light scattering

Griffiths, Peter Charles, Cattoz, Beatrice, Ibrahim, Mervat Shafik and Anuonye, Josephine Chibuzor (2015) Probing the interaction of nanoparticles with mucin for drug delivery applications using dynamic light scattering. European Journal of Pharmaceutics and Biopharmaceutics, 97 (A). pp. 218-222. ISSN 0939-6411 (doi:10.1016/j.ejpb.2015.05.004)

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Abstract

Drug delivery via the eye, nose, gastrointestinal tract and lung is of great interest as they represent patient-compliant and facile methods to administer drugs. However, for a drug to reach the systemic circulation it must penetrate the “mucus barrier”. An understanding of the characteristics of the mucus barrier is therefore important in the design of mucus penetrating drug delivery vehicles e.g. nanoparticles. Here, a range of nanoparticles – silica, aluminium coated silica, poly (lactic-co-glycolic acid) (PLGA) and PEGylated PLGA – each with known but different physicochemical characteristics were examined in the presence of mucin to identify those characteristics that engender nanoparticle/mucin interactions and thus, to define “design rules” for mucus penetrating (nano)particles (MPP), at least in terms of the surface characteristics of charge and hydrophilicity. Dynamic light scattering (DLS) and rheology have been used to assess the interaction between such nanoparticles and mucin. It was found that negatively charged and hydrophilic nanoparticles do not exhibit an interaction with mucin whereas positively charged and hydrophobic nanoparticles show a strong interaction. Surface grafted poly (ethylene glycol) (PEG) chains significantly reduced this interaction. This study clearly demonstrates that the established colloid science techniques of DLS and rheology are very powerful screening tools to probe nanoparticle/mucin interactions.

Item Type: Article
Additional Information: This is the Author's Accepted Manuscript version, uploaded in accordance with the publisher's self-archiving policy. Please note: this is a PDF file of an unedited manuscript that has been accepted for publication. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. During the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Uncontrolled Keywords: mucin, silica, aluminium coated silica, poly (lactic-co-glycolic acid) (PLGA), PEGylated PLGA, dynamic light scattering, rheology
Subjects: Q Science > QD Chemistry
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Department of Pharmaceutical, Chemical & Environmental Sciences
Last Modified: 17 Oct 2016 09:13
URI: http://gala.gre.ac.uk/id/eprint/13630

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