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Prophylactic effect of methyl-3-O-methyl gallate against sodium fluoride-induced oxidative stress in rat’s erythrocytes in vivo.

Prophylactic effect of methyl-3-O-methyl gallate against sodium fluoride-induced oxidative stress in rat’s erythrocytes in vivo.

Nabavi, S.M., Habtemariam, Solomon, Nabavi, S.F., Moghaddam, A.H. and Latifi, A.M. (2013) Prophylactic effect of methyl-3-O-methyl gallate against sodium fluoride-induced oxidative stress in rat’s erythrocytes in vivo. Journal of Pharmacy and Pharmacology, 65 (6). pp. 868-873. ISSN 0022-3573 (Print), 2042-7158 (Online) (doi:10.1111/jphp.12048)

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Abstract

Objectives

The purpose of this study was to demonstrate the effect of methyl-3-O-methyl gallate (M3OMG), a rare polyphenolic natural product with a potent in-vitro antioxidant effect, against sodium fluoride (NaF)-induced oxidative stress in rat erythrocytes in vivo.

Methods

Male Wistar rats were treated daily with either M3OMG (10 and 20 mg/kg) obtained through synthesis, vitamin C (10 mg/kg) or vehicle intraperitoneally for 7 days. Oxidative stress was then induced by exposing animals to NaF (600 ppm) through drinking water for 7 days. At the end of intoxication period, rats were killed and erythrocytes isolated. The activity of antioxidant enzymes (catalase and superoxide dismutase) and levels of reduced glutathione and thiobarbituric acid reactive substances were measured in erythrocyte haemolysates.

Results

NaF intoxication resulted in a 1.9-fold increase in erythrocyte lipid peroxidation associated with significant (P < 0.001) depletion of reduced glutathione level. Superoxide dismutase and catalase activity was suppressed by NaF treatment by 3.069 and 2.3 fold when compared with untreated control groups. Pretreatment of rats with M3OMG or vitamin C afforded protection against NaF-induced oxidative stress as assessed through the measured oxidant/antioxidant markers.

Conclusion

This finding provided in-vivo evidence for the therapeutic potential of M3OMG in combating fluoride-induced oxidative damage in cellular systems.

Item Type: Article
Uncontrolled Keywords: Erythrocyte, Fluoride, Methyl-3-O-methyl gallate, Oxidative damage
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Pharmaceutical, Chemical & Environmental Sciences
Last Modified: 17 Oct 2016 09:13
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/13425

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