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Action of iron and fructose on gene expression in human liver cells

Action of iron and fructose on gene expression in human liver cells

Christides, Tatiana and Sharp, Paul (2013) Action of iron and fructose on gene expression in human liver cells. In: BioIron Abstracts: American Journal of Hematology. Wiley-Blackwell, London, UK, E232-E232. ISSN 1096-8652 (doi:10.1002/ajh.23453)

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Abstract

Introduction: Evidence suggests that iron and fructose may play a role in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) – the most common liver disorder in the developed world. Furthermore, there is evidence that chemical interactions between iron and fructose generate hepatotoxic metabolites that could contribute to NAFLD progression; however, other studies have shown that fructose sequesters iron and is protective against iron-induced oxidative stress. Previously we showed fructose increased liver iron uptake. The aim of this study was to investigate the effects of iron and fructose - alone and in combination - on gene expression in human liver cells.
Methods: HepG2 hepatoma cells were treated with 1μM ferric ammonium citrate (FAC) and 15 mM fructose for 24 hours, and then harvested for RNA. Gene expression was assessed using Affymetrix Genechip Human Genome microarray and subsequent bioinformatic analysis using GeneGo Metacore software.
Results: Treatment of cells with fructose and FAC together decreased the expression of signalling proteins in two major pathways implicated in development of inflammatory liver disease: transforming growth factor β (TGF β) - as evidenced by lowered gene expression of SMAD2 and SMAD3 - and STAT3/NF-κB. Fructose treatment alone activated complement pathways with increased expression of C3, C5 convertase and the membrane attack complex.
Discussion and Conclusion: Our results suggest that hepatic fructose-iron interactions are anti-inflammatory, possibly via fructose induced iron sequestration; fructose alone, however, may cause inflammation by increasing activity of the innate immune system. The liver is continually exposed to both iron and fructose via the portal circulation system. Further research is essential to assess the roles of iron and fructose metabolism in the development and progression of NAFLD.

Item Type: Conference Proceedings
Title of Proceedings: BioIron Abstracts: American Journal of Hematology
Additional Information: [1] Copyright: © Wiley Periodicals, Inc. [2] Poster #334 presented by Tatiana Christides at the BioIron 2013: 5th Meeting of the International BioIron Society, 14—18 April 2013, University College London, UK
Uncontrolled Keywords: iron, fructose, NAFLD
Subjects: Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
R Medicine > RC Internal medicine > RC1200 Sports Medicine
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Department of Life & Sports Sciences
Related URLs:
Last Modified: 17 Oct 2016 09:12
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/12352

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