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Evaluation of non-polar bonded-phases for the clean-up of maize extracts prior to aflatoxin assay by HPTLC

Evaluation of non-polar bonded-phases for the clean-up of maize extracts prior to aflatoxin assay by HPTLC

Tomlins, K.I., Jewers, K. and Coker, R.D. (1989) Evaluation of non-polar bonded-phases for the clean-up of maize extracts prior to aflatoxin assay by HPTLC. Chromatographia, 27 (1-2). pp. 67-70. ISSN 0009-5893 (Print), 1612-1112 (Online) (doi:10.1007/BF02290408)

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Abstract

The ability of a PH non-polar bonded phase to extract aflatoxin from aqueous solutions (1% v/v) containing 5, 10, 20 and 30% acetone, acetonitrile or methanol was determined. The amount of aflatoxin extracted by the cartridge from aqueous solutions remained almost constant (97–102%) as the concentration of methanol increased, but decreased rapidly when the amount of acetone or acetonitrile increased beyond 10%. Examination of the ability of a number of bonded phases (C2, C8, C18, CH and PH) to extract aflatoxin from aqueous methanol extracts of maize revealed that the PH bonded phase was the most efficient. Aflatoxin is eluted from the cartridge by chloroform (7 ml). An additional clean-up step involving bi-directional development was required when quantifying low levels of aflatoxin in maize by HPTLC.

Item Type: Article
Uncontrolled Keywords: aflatoxin, non-polar bonded phase extraction cartridges, high performance thin layer chromatography
Subjects: Q Science > QD Chemistry
S Agriculture > S Agriculture (General)
Faculty / Department / Research Group: Faculty of Engineering & Science > Natural Resources Institute
Related URLs:
Last Modified: 06 Aug 2014 14:53
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/11919

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