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Construction and physiochemical characterisation of a multi-composite, potential oral vaccine delivery system (VDS)

Construction and physiochemical characterisation of a multi-composite, potential oral vaccine delivery system (VDS)

Pettit, Marie, Dyer, Paul D.R., Mitchell, John C. ORCID: 0000-0003-2945-3292, Griffiths, Peter C. ORCID: 0000-0002-6686-1271, Alexander, Bruce, Cattoz, Beatrice, Heenan, Richard K., King, Stephen M., Schweins, Ralf, Pullen, Frank, Wicks, Stephen R. and Richardson, Simon C.W. (2014) Construction and physiochemical characterisation of a multi-composite, potential oral vaccine delivery system (VDS). International Journal of Pharmaceutics, 468 (1-2). pp. 264-271. ISSN 0378-5173 (doi:10.1016/j.ijpharm.2014.03.046)

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Abstract

An increasing human population requires a secure food supply and a cost effective, oral vaccine delivery system for livestock would help facilitate this end. Recombinant antigen adsorbed onto silica beads and coated with myristic acid, was released (∼15% (w/v)) over 24 h at pH 8.8. At pH 2, the myristic acid acted as an enteric coating, protecting the antigen from a variety of proteases. The antigen adsorbed onto silica particles, coated in myristic acid had a conserved secondary structure (measured by circular dichroism (CD) spectroscopy) following its pH-triggered release. Small angle neutron scattering (SANS) was used to measure the thickness of the adsorbed antigen, finding that its adsorbed conformation was slightly greater than its solution radius of gyration, i.e. 120–160 Å. The addition of myristic acid led to a further increase in particle size, with scattering data consistent with an acid thickness slightly greater than a monolayer of fully extended alkyl chains and a degree of hydration of around 50%. Whilst adsorbed onto the silica and coated in myristic acid, the protein was stable over 14 days at 42 °C, indicating a reduced need for cold chain storage. These data indicate that further investigation is warranted into the development of this technology.

Item Type: Article
Additional Information: [1] The full-text version attached is made available under License: http://creativecommons.org/licenses/by-nc-nd/3.0/. Please cite this article in press as: Pettit, M.W., et al., Construction and physiochemical characterisation of a multi-composite, potential oral vaccine delivery system (VDS), Int J Pharmaceut (2014), http://dx.doi.org/10.1016/j.ijpharm.2014.03.046. [2] Appendix A. Supplementary data: Supplementary data associated with this article can be found, in the online version, at http://dx.doi.org/10.1016/j.ijpharm.2014.03.046. [3] Acknowledgements (funding): MWP would like to thank the University of Greenwich for her PhD scholarship. SCR would like to acknowledge grant RB1320038 from the Science and Technology Facilities Council (STFC).
Uncontrolled Keywords: APCs, BCA, CD,Enteric, GST–GFP, IPTG, immobilisation, LP, MALDI-TOF, MDA, PP, Peyer’s patch, SANS, SDS PAGE, silica, synthetic protein, VDS, vaccine, antigen presenting cells, bicinchoninic acid assay, circular dichroism, glutathione-S-transferase (GST), lamina propria, maternally derived antibodies, matrix assisted laser desorption ionisation time o, small angle neutron scattering, sodium dodecyl sulphate polyacrylamide gel electro, vaccine delivery systemsenteric, immobilisation
Subjects: R Medicine > RS Pharmacy and materia medica
Faculty / Department / Research Group: Faculty of Engineering & Science
Faculty of Engineering & Science > Department of Life & Sports Sciences
Related URLs:
Last Modified: 14 Mar 2018 07:22
Selected for GREAT 2016: GREAT b
Selected for GREAT 2017: None
Selected for GREAT 2018: GREAT c
URI: http://gala.gre.ac.uk/id/eprint/11402

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