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Secondary kinetic isotope effects as probes of environmentally-coupled enzymatic hydrogen tunneling reactions

Secondary kinetic isotope effects as probes of environmentally-coupled enzymatic hydrogen tunneling reactions

Hay, Sam, Pang, Jiayun, Monaghan, Phillip J., Wang, Xi, Evans, Rhiannon M., Sutcliffe, Michael J., Allemann, Rudolf K. and Scrutton, Nigel S. (2008) Secondary kinetic isotope effects as probes of environmentally-coupled enzymatic hydrogen tunneling reactions. ChemPhysChem, 9 (11). pp. 1536-1539. ISSN 1439-4235 (Print), 1439-7641 (Online) (doi:10.1002/cphc.200800291)

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Abstract

The secondary kinetic isotope effect for hydride transfer from NADPH to dihydrofolate catalyzed by dihydrofolate reductase (see traces) is neither temperature dependent nor exalted. In environmentally coupled models of H-tunneling, the secondary isotope effects do not report on promoting motions, but reflect the active site geometry attained immediately prior to H transfer (i.e. the ‘tunnelling ready configuration′).

Item Type: Article
Additional Information: [1] Journal published on behalf of ChemPubSoc Europe.
Uncontrolled Keywords: secondary kinetic isotope effect, hydrogen transfer, dihydrofolate reductase
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Pharmaceutical, Chemical & Environmental Sciences
Related URLs:
Last Modified: 17 Oct 2016 09:12
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/10616

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