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Molecular genetics of 2, 4-dichlorophenoxyacetate (2, 4-D) degradation in pseudomonas cepacia strain 2a

Molecular genetics of 2, 4-dichlorophenoxyacetate (2, 4-D) degradation in pseudomonas cepacia strain 2a

Aathithan, Sivasampu (1992) Molecular genetics of 2, 4-dichlorophenoxyacetate (2, 4-D) degradation in pseudomonas cepacia strain 2a. MPhil thesis, Thames Polytechnic.

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Abstract

Pseudomonas cepacia strain 2a, which utilises2,4-dichlorophenoxyacetate (2,4-D) as a sole source of carbon and energy, has been shown to contain an 17kbp plasmid, p2,4-D, which has been mapped using BamHI and Asp718. Several mutants of strain 2a unable to grow on 2,4-D were generated by temperature curing. All bore plasmids of diminished size (57Kbp), created by deletion of 30Kbp from the parental plasmid. These were called p2,4-Del. The plasmid p2,4-Del was presumed to possess the origin of replication and related sequences but had lost some or all of the genes encoding enzymes for 2,4-D degradation.

Item Type: Thesis (MPhil)
Uncontrolled Keywords: molecular genetics, biology
Subjects: Q Science > QH Natural history
Faculty / Department / Research Group: Faculty of Engineering & Science > Department of Life & Sports Sciences
Last Modified: 17 Oct 2016 09:12
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/10334

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