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The physiology of sterol nutrition in the pea aphid Acyrthosiphon pisum

The physiology of sterol nutrition in the pea aphid Acyrthosiphon pisum

Bouvaine, Sophie, Behmer, Spencer T., Lin, George G., Faure, Marie-Line, Grebenok, Robert J. and Douglas, Angela E. (2012) The physiology of sterol nutrition in the pea aphid Acyrthosiphon pisum. Journal of Insect Physiology, 58 (11). pp. 1383-1389. ISSN 0022-1910 (doi:10.1016/j.jinsphys.2012.07.014)

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Abstract

The phloem sap of fava bean (Vicia faba) plants utilized by the pea aphid Acyrthosiphon pisum contains three sterols, cholesterol, stigmasterol and sitosterol, in a 2:2:1 ratio. To investigate the nutritional value of these sterols, pea aphids were reared on chemically-defined diets containing each sterol at 0.1, 1 and 10 μg ml−1 with a sterol-free diet as control. Larval growth rate and aphid lifespan did not vary significantly across the diets, indicating that sterol reserves can buffer some performance indices against a shortfall in dietary sterol over at least one generation. However, lifetime reproductive output was depressed in aphids on diets containing stigmasterol or no sterol, relative to diets supplemented with cholesterol or sitosterol. The cholesterol density of embryos in teneral adults was significantly higher than in the total body; and the number and biomass of embryos in aphids on diets with stigmasterol and no sterols were reduced relative to diets with cholesterol or sitosterol, indicating that the reproductive output of the pea aphid can be limited by the amount and composition of dietary sterol. In a complementary RNA-seq analysis of pea aphids reared on plants and diets with different sterol contents, 7.6% of the 17,417 detected gene transcripts were differentially expressed. Transcript abundance of genes with annotated function in sterol utilization did not vary significantly among treatments, suggesting that the metabolic response to dietary sterol may be mediated primarily at the level of enzyme function or metabolite concentration.

Item Type: Article
Additional Information: [1] Supplementary data associated with this article can be found, in the online version, at http://dx.doi.org/10.1016/j.jinsphys.2012. 07.014.
Uncontrolled Keywords: Acyrthosiphon pisum, aphid, cholesterol, sterol, phloem sap, phytosterol
Subjects: Q Science > QH Natural history
S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Faculty / Department / Research Group: Faculty of Engineering & Science > Natural Resources Institute
Related URLs:
Last Modified: 11 Sep 2014 11:18
Selected for GREAT 2016: None
Selected for GREAT 2017: None
Selected for GREAT 2018: None
URI: http://gala.gre.ac.uk/id/eprint/10301

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